饲料中益生菌对凡纳滨对虾生长、肠道菌群及部

【作者】 胡毅； 谭北平； 麦康森； 艾庆辉； 郑石轩； 程开敏；

【Author】 HU Yi1,TAN Bei-ping1,MAI Kang-sen1,AI Qing-hui 1,ZHENG Shi-xuan2,CHENG Kai-min2 (1.The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China;2.Zhanjiang Yuehai Feed Co.Ltd.,Zhanjiang 524017,China)

【机构】 中国海洋大学教育部海水养殖重点实验室； 湛江粤海饲料有限公司； 湛江粤海饲料有限公司 山东青岛266003； 山东青岛266003； 广东湛江524017；

【摘要】 以初体质量为(0.11±0.00)g的凡纳滨对虾(Litopenaeus vannamei)为研究对象,在室外水泥池进行10周饲喂实验,研究饲料中添加单一益生菌和复合益生菌对凡纳滨对虾生长、消化酶、肠道和粪便菌群及部分免疫指标的影响。以基础饲料为对照组,在基础饲料中分别添加地衣芽孢杆菌(Bacillus licheniformis)、枯草芽孢杆菌(Bacillus subtilis)、虾乐333(复合益生菌)、地衣芽孢杆菌+枯草芽孢杆菌(1∶1)、虾乐333+地衣芽孢杆菌+枯草芽孢杆菌(1∶1∶1)各1g/kg,配制5种实验饲料。实验结果显示,复合益生菌饲喂组对虾的特定生长率显著高于对照组(P<0.05),而单一益生菌饲喂组与对照组差异不显著(P<0.05)。饲料中添加益生菌能使凡纳滨对虾肠道蛋白酶和淀粉酶活力升高,其中复合益生菌饲喂组淀粉酶活力显著高于对照组(P<0.05)。与对照组比,饲料中添加益生菌显著降低了肠道及粪便弧菌数(P<0.05),不同程度提高了凡纳滨对虾血清蛋白浓度、酚氧化酶活力、溶菌酶活力和总抗氧化力,其中添加复合益生菌能使血清蛋白浓度及溶菌... 更多

【Abstract】 A 10-week feeding experiment was conducted to evaluate the effects of single and pound probiotic on growth,digestive enzyme activities of intestine,bacteria of intestine and fecal, and immunity of juvenile Litopenaeus vannamei [initial body weight (0.11 ± 0.00)g]. Basic diet was made of fish meal and defatted soybean meal as main protein sources,menhaden fish oil and soybean lecithin oil as lipid source,wheat meal as the carbohydrate source. Shrimps were fed six different diets: control (nons... 更多